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Primers for qRT-PCR analysis of TWNK and mtDNA copy number.
| Gene | Primer sequence (5′–3′) | Size (bp) | Temperature (°C) |
| TWNK | F-TGACCTTCCATGGCCAACAG | 168 | 56 |
| R-AGGCTCCGACGATGAAATCC | |||
| β-actin | F-GATATTGCTGCGCTCGTTG | 178 | 56 |
| R-TTCAGGGTCAGGATACCTCTTT | |||
| tRNA-Leu | F-GCTCGGCAAATGCAAAAGG | 50 | 56 |
| R-AGGATTTGAACCTCTGGATAAAGGG | |||
| rRNA-16S | F-TGCGTCAAAGCTCCCTCATT | 128 | 56 |
| R-ACGCCGTAGGAGGATAGGTT | |||
| β2M | F-TCCTTCAACGACGACTGGAC | 132 | 56 |
| R-CCGTACCCCACTTGTAGACC |
