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Primers for vector construction of pcDNA3.1-TWNK wt and pcDNA3.1-TWNK A137T.
| Gene | Primer sequence (5′–3′) | Size (bp) | Temperature (°C) |
| pcDNA3.1-TWNK wt | F-CCCAAGCTTATGGCGGCGGTG | 2,010 | 56 |
| R-CCGGAATTCTCAGGGCTTGCTGGA | |||
| TWNK mut | F-GGCACCGCGGGGTCCCGACGCCCG GCCCTGACGAG | 4,702 | 58 |
| R-TCAGGGCCGGGCGTCGGGACCCCG CGGTGCCGCAG |
