Skip to main content
. 2020 Jul 14;11:1468. doi: 10.3389/fimmu.2020.01468

Figure 1.

Figure 1

CXCR4 and CXCL12 expression vary during bursa development. (A) At the indicated time points, RNA from total bursa (organ pools at ED10, individual organs all other time points; n = 5) was analyzed for CXCL12 and CXCR4 expression by qRT-PCR; (B) B cells were sort purified from ED18 and D2 bursa. RNA from biological replicates of B cells isolated from pooled bursae (ED18 n = 4; D2 n = 3) was analyzed by qRT-PCR for CXCL12 and CXCR4 expression in comparison to RNA from total bursa RNA (ED18 n = 4; D2 n = 3). (A,B) Shown are mean and SD. Significance was calculated by One-Way-ANOVA and Bonferroni post-hoc test with *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001 and refers in (A) within the analysis of CXCL12 or CXCR4 to the preceding time point and in (B) to the respective ED18 bursa value.