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Characterization of CXCR4 low cells. Leukocytes from a 4-weeks old bursa and PBL were stained for (A) chB6, CXCR4, and Light chain or MHC class II or (B) chB6, CXCR4, and 11A9 and analyzed by flow cytometry. Histograms in (A) are gated for chB6+/CXCR4-low (blue) or chB6+/CXCR4-high (gray) bursal B cells and chB6+ blood B cells (red). Dot plots in (B) are gated for chB6+ cells.
