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. 2020 Jul 14;11:1553. doi: 10.3389/fmicb.2020.01553

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Copyright © 2020 Kappler, Lasanajak, Smith, Opitz and Hennet.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Increased IgG reactivity and decreased IgG repertoire to commensal bacteria in the blood serum of CD patients. (A) Percentage of IgG-positive cells of an intestinal complex microbiota, determined by flow cytometry, as the result of three independent experiments (different batches of bacterial culture and different serum samples, n = 20 for ctrl, n = 17 for UC, n = 21 for CD). (B) Alpha-diversity, shown as the number of different OTUs within one sample, grouped by control, UC and CD, resulting from sorting and 16S sequencing of IgG-bound bacteria of 6 samples per group. *p-value ≥ 0.05, determined using one-way ANOVA and Bonferroni’s post hoc test with selected pairs for ctrl vs UC and ctrl vs CD.