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. 2020 Jul 14;11:1553. doi: 10.3389/fmicb.2020.01553

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Copyright © 2020 Kappler, Lasanajak, Smith, Opitz and Hennet.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bacteroides surface glycans recognized by fucose-specific lectin AAL. (A) Flow cytometry histograms of Bacteroides species and E. coli K12 stained with fluorescein-AAL (red) compared with unstained bacteria (black). (B) Analysis of bacterial cell lysates by SDS-PAGE and Coomassie (loading control) and by lectin blotting (AAL). The specificity of the AAL signal was confirmed using competing soluble L-fucose (AAL + Fucose). (C) Analysis of isolated LPS by SDS-PAGE and silver stain (loading control) and by lectin blotting (AAL). The specificity of AAL was tested with the inhibiting sugar L-fucose (AAL + Fucose). Ctrl, E. coli O127:B8; 1, E. coli K12; 2, B. intestinalis; 3, B. thetaiotaomicron; 4, B. vulgatus; 5, B. stercoris; 6, B. acidifaciens.