TABLE 2.
Primer for 16S sequencing of V3V4 region.
| name | Nextera adapter | linker | shift | primer site (5′–3′) |
| 341F_nex0 | TCGTCGGCAGCGTC | AGATGTGTATAAGAGACAG | T | CCTACGGGNGGCWGCAG |
| 341F_nex1 | TCGTCGGCAGCGTC | AGATGTGTATAAGAGACAG | NT | CCTACGGGNGGCWGCAG |
| 341F_nex2 | TCGTCGGCAGCGTC | AGATGTGTATAAGAGACAG | NNT | CCTACGGGNGGCWGCAG |
| 341F_nex3 | TCGTCGGCAGCGTC | AGATGTGTATAAGAGACAG | NNNT | CCTACGGGNGGCWGCAG |
| 802R_nex0 | GTCTCGTGGGCTCGG | AGATGTGTATAAGAGACAG | T | TACNVGGGTATCTAATCC |
| 802R_nex1 | GTCTCGTGGGCTCGG | AGATGTGTATAAGAGACAG | NT | TACNVGGGTATCTAATCC |
| 802R_nex2 | GTCTCGTGGGCTCGG | AGATGTGTATAAGAGACAG | NNT | TACNVGGGTATCTAATCC |
| 802R_nex3 | GTCTCGTGGGCTCGG | AGATGTGTATAAGAGACAG | NNNT | TACNVGGGTATCTAATCC |