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. 2020 Jul 13;7:351. doi: 10.3389/fvets.2020.00351

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Copyright © 2020 Ding, Li, Jiang, Li, Zhang, Song, Hayat, Zhang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gross sections of the lamellae layer from (A,B) control and (C,D) oligofructose treated heifers. Labels in the sections: B, basal cells; SB, surprabasal cells; BM, basement membrane; DL, dermal lamellae; EL, epidermal lamellae; WBC, white blood cells; Tip, the tip of the epidermal lamellae). (A) H&E stain. The normal appearance of the dermo-epidermal junction consists of numerous interlocking lamellae. (B) Periodic acid–Schiff (PAS) stain. The tip of the epidermal lamellae is rounded and in a close association with the basement membrane. (C) H&E stain. Stretched epidermal lamellae and increased numbers of suprabasal cells are observed. (D) PAS stain. The basement membrane has an attenuated appearance; the basal cells are enlarged and wider in the dermal lamellae.