Table 2.

Gene, sequence, amplification size, and efficiency of the primers used for quantitative PCR (qPCR).

Gene	Polarity	Sequence (5′ → 3′)	Size (bp)	Amplification efficiency (%)	NCBI accession no.
Cytokine
IL-1β	Forward	ATTCTCTCCAGCCAACCTTCATT	100	96	NM_174093
	Reverse	TTCTCGTCACTGTAGTAAGCCATCA
IL-6	Forward	ATGACTTCTGCTTTCCCTACCC	180	105	NM_173923
	Reverse	GCTGCTTTCACACTCATCATTC
IL-8	Forward	GACAGCAGAGCTCACAAGCATCT	105	94	NM_173925.2
	Reverse	AAGCTGCCAAGAGAGCAACAG
IL-10	Forward	ACAGGCTGAGAACCACGGGC	175	90	NM_174088.1
	Reverse	GACACCCCTCTCTTGGAGCTCACT
TNF-α	Forward	CCAGAGGGAAGAGCAGTCCC	114	92	NM_173966.3
	Reverse	TCGGCTACAACGTGGGCTAC
Chemokine
CXCL-1	Forward	CATCCAGAGCGTGAAGGTGA	100	97	NM_175700.2
	Reverse	GGTGGGGTTGAGACACACTT
CXCL-6	Forward	TGAGAGAGCTGCGTTGTGTG	119	103	NM_174300.2
	Reverse	GGTGGCTATCACTTCCACCT
MCP-1	Forward	GCAATTAACTCCCAAGTCGCC	161	95	NM_174006.2
	Reverse	TGCTTGGGGTCTGCACATAA
MCP-2	Forward	ATCACCAACAGCCAGTGTCC	133	91	NM_174007.1
	Reverse	TCGGTGTTCGGGACTTTTGG
Adhesion molecule
ICAM-1	Forward	CGACCACAGGAGCAACTTCT	171	106	NM_174348.2
	Reverse	TCGCACTTCAGGGTCTGTTC
E-selectin	Forward	CATCCTCAGAACGGCACTGT	167	97	NM_174181.2
	Reverse	ACTTCACAAACTGGGACCCG
Inflammatory molecule
COX-2	Forward	ATCTACCCGCCTCATGTTCCT	187	93	AF031698
	Reverse	GGATTAGCCTGCTTGTCTGGA
iNOS	Forward	CAGGATGACCCCAAACGTCA	190	101	XM_024979646.1
	Reverse	CCTTCTGGTGAAGCGTGTCT
PAI-1	Forward	TCTTCCACAAGTCCGATGGC	142	92	XM_024984644.1
	Reverse	ATGCTGAGAGTGTTCCCGTG
Housekeeping gene
ACTB	Forward	ACTTGCGCAGAAAACGAGAT	123	97	BT030480
	Reverse	CACCTTCACCGTTCCAGTTT
GAPDH	Forward	GGGTCATCATCTCTGCACCT	176	95	DQ402990
	Reverse	GGTCATAAGTCCCTCCACGA
UXT	Forward	TGTGGCCCTTGGATATGGTT	101	101	BC108205.1
	Reverse	GGTTGTCGCTGAGCTCTGTG