Figure 6.

SBP promotes expressions of NO, NOS, AChE, and cytokine in APP/PS1 transgenic mice. Treatment of mice was as described in Figure 2 . For determination of levels of NO, NOS, and AChE, 0.1 g of brain tissue was homogenized using 0.9 ml of pre-cold saline. After reacted on ice for 15 min, the homogenate was centrifuged at 2,500 rpm/min for 10 min at 4°C, and the supernatants were used to measure the levels of NO, NOS, and AChE with application of corresponding kits according to the manufacturer’s instructions. Blood was collected for the measurement of the amounts of IL-6 and TNF-α. The blood was centrifuged at 3,500 rpm/min for 10 min, and the serum was obtained by discarding the precipitant. The levels of IL-6 and TNF-α in the serum was further determined using commercially available ELISA kits. Data are expressed as Mean ± SEM of the percentage of change as compared with control (untreated wild type mice), where n = 6; p < 0.05 (*); p < 0.01 (**); p < 0.001 (***) vs control group. p < 0.05 (#); p < 0.01 (##); p < 0.001 (###) vs model group.