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. 2020 Jul 10;11:1630. doi: 10.3389/fmicb.2020.01630

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Copyright © 2020 Zhu, Lv, Fang, Peng, Sheng, Xiao, Kumar Ojha, Yan, Liao and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HSPA8 interacts with the N-terminal 19–272 amino acids of S1 subunit of IBV Beaudette, H120 and QX strains and M41-RBD interacts with HSPA8 of human and pig. (A–C) Detection of interaction between chicken HSPA8 and the N-terminal 19–272 amino acids of the S1 subunit of Beaudette (A), H120 (B), and QX (C) strains. 293T cells were cotransfected with indicated plasmids and then flag-tagged HSPA8 was immunoprecipitated with anti-flag antibody. The precipitated proteins were detected by Western blotting using anti-flag and anti-GFP antibodies. (D) Detection of the interaction between IBV M41-RBD and HSPA8 of human and pig. 293T cells were cotransfected with indicated plasmids and then flag-tagged HSPA8 was immunoprecipitated with anti-flag antibody. The precipitated proteins were detected by Western blotting using anti-flag and anti-GFP antibodies.