FIGURE 1.

Cardinal temperatures for Bacillus firmus I-1582 growth and biofilm formation. (A) Number of bacterial CFU after 96 h of incubation in solid nutrient agar at different temperatures. Each value is mean ± standard error of three replicates. (B) Bacterial growth kinetics incubated for 0, 2, 4, 6, 8, 10, 12, 24, 30, 36, and 48 h in Luria-Bertani (LB) liquid media at different temperatures. Each value is mean ± standard error of three replicates. (C) Biofilm formation in Schaeffer’s sporulation medium plus glucose and glycerol (SGG) media 48 h after being inoculated with 10⁶ bacterial CFU and incubated at different temperatures.