FIGURE 6.
Dynamics of the relative gene expression of genes related to the jasmonic acid (Lox D) and the salicylic acid (PR1/Pal I) pathways in tomato (A–C) and cucumber (D–F) plants that were non-inoculated (Control), inoculated with Bacillus firmus I-1582 (Bf), inoculated with Meloidogyne incognita (Mi) and co-inoculated with both nematode and bacteria (Mi+Bf). Bf I-1582 treatments were inoculated with 109 CFU 1 week after transplanting, while M. incognita treatments were inoculated with 200 juveniles 2 weeks after transplanting. The relative expression of the genes was evaluated at 0 days after nematode inoculation (DANI), at 7 and at 40 DANI. The ubiquitin (UBI) gene was used as a reference gene for both plant species. Each value is mean ± standard error of three biological samples with two technical replicates each. Each biological replicate consisted of the roots from three plants pooled together. Asterisk indicates significant differences with respect to the Control using the non-parametric Wilcoxon test (P< 0.05).