Figure 6.

Treatment of Se-D mice with 15d-PGJ₂ increases ILC3 and Th17 cells PI. Se-D mice were treated with 15d-PGJ₂ at 0.05 mg/kg intraperitoneally on day −1 PI followed by daily injections. (A) Shedding curve of C. rodentium infected Se-D mice treated with control or 15d-PGJ₂. (B) Colon length of Se-D mice treated with control or 15d-PGJ₂ on day 11 PI. (C) Colon length of Se-D mice treated with control or 15d-PGJ₂ on day 21 PI. (D) Colonic crypt length of Se-D mice treated with control or 15d-PGJ₂ on day 11 PI. (E) Representative flow plot and (F) quantification of ILC3s isolated from the cLP on day 11 PI. Gray box around representative flow plots indicate IL-17A⁺IL-22⁺ cells. (G) Representative flow plot and (H) quantification of IL-17A⁺ cells of CD4⁺ T cells indicating Th17 cells isolated from the cLP on day 11 PI. Gray box around representative flow plots indicate IL-17A⁺ cells. Data indicate mean ± SEM of two independent experiments and n = 4–8 mice/group, unpaired t-test (B,C,E,G) ^*P < 0.05, ^**P < 0.01, n.s., not significant.