Figure 8.

Lack of selenoprotein expression in macrophages decreases survival following infection. (A) Survival curve of Trsp^fl/fl WT and Trsp^fl/fl LysM^Cre (Trsp KO) mice on Se-D and Se-A diets. (B) Shedding curve of Trsp^fl/fl WT and Trsp KO mice on both Se-D and Se-A diet. (*comparing Trsp WT on Se-D vs. Trsp WT on Se-A at day 14; **comparing Trsp WT Se-D vs. Trsp KO Se-A at day 14) (C) Percent weight change of Trsp WT and Trsp KO mice on Se-D and Se-A diet. $ represents *comparing Trsp WT on Se-D vs. Trsp WT on Se-A on days 2, 9 PI; *comparing Trsp KO on Se-D vs. Trsp WT on Se-A on days 2, 9, 21, 25, 28, and 30 PI; **comparing Trsp KO on Se-D vs. Trsp WT on Se-A on day 11 PI. (D) Colon length of Trsp WT and Trsp KO mice on Se-D and Se-A diet on day 11 PI. (E) Representative flow plot and (F) quantification of ILC3s isolated from the cLP on day 11 PI. Gray box around representative flow plots indicate IL-17A⁺IL-22⁺ cells. (G) Representative flow plot and (H) quantification of IL-17A⁺ cells of CD4⁺ T cells indicating Th17 cells isolated from the cLP on day 11 PI. Gray box around representative flow plots indicate IL-17A⁺ cells. Data indicate mean ± SEM of two independent experiments and n = 3-9 mice/group, two-way ANOVA (B,C), one-way ANOVA (D,F,H). *P < 0.05, **P < 0.01, n.s., not significant.