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. 2020 Jul 9;8:779. doi: 10.3389/fbioe.2020.00779

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Copyright © 2020 He, Chen, Wan, Zhao, Qiu, Lin, Zhang and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytotoxicity of the decellularized meniscus ECM scaffold and recellularization in vitro. (A) Cytotoxicity assay of DM: the proliferative activities of the cells cultured in standard medium and those cultured in the extracts at different concentrations. (B) Fluorescence Live-Dead cell (Scale bar: 30 μm) and DAPI (Scale bar: 100 μm) staining images (two and three dimensions) demonstrating seeding of BMSCs on DM ECM scaffolds at 2 weeks.