Skip to main content
. Author manuscript; available in PMC: 2020 Dec 24.
Published in final edited form as: Nature. 2020 Jun 24;583(7817):609–614. doi: 10.1038/s41586-020-2422-6

Extended Data Figure 10. Ex vivo characterization of a human DR-18 variant.

Extended Data Figure 10.

(a) SPR sensorgrams of human DR-18 (hDR-18) binding to human or cynomolgus macaque (Macaca fascicularis) IL-18Rα and IL-18BP. (b) Concentration-response curves of hIL-18 or hDR-18 on HEK Blue IL-18R reporter cells. (c) Titration of hIL-18BP on a fixed concentration of hIL-18 (1 ng/mL) or hDR-18 (0.1 ng/mL) on HEK Blue IL-18R reporter cells. (d) Concentration-response curves of IFN-γ stimulation by hIL-18 or hDR-18 on human peripheral blood mononuclear cells (PBMCs) or (e) cynomolgus macaque PBMCs. IFN-γ concentration is measured by ELISA. Data are representative of at least 2 independent experiments with n=2 for (b-e) and presented as the mean ± SEM.