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. Author manuscript; available in PMC: 2021 Jul 22.
Published in final edited form as: Neuron. 2020 May 11;107(2):274–282.e6. doi: 10.1016/j.neuron.2020.04.018

Figure 3. Projection- and Layer-dependent Input Tracing of V1→AL and V1→PM Neurons.

Figure 3.

(A) Schematic showing the experimental design on projection- and layer-dependent monosynaptic input tracing by G-deleted rabies (left and middle). Two possible feedback connectivity models depending on projection (right). (B) Intrinsic signal imaging to define higher visual area borders. (C) Reconstruction of rabies-traced and blood vessel stained visual cortex using light sheet microscopy. (D) Summary of long-range feedback inputs onto V1→AL and V1→PM neurons in L2/3 (left) or L5 (right). (E) Summary of long-range feedback inputs onto V1→AL, V1→PM, and V1→P/POR neurons. Values are reported as mean ± SEM. Statistics were calculated from two-way ANOVAs with Sidak’s or Turkey’s post-hoc multiple-comparison tests for panels D and E, respectively. *p<0.05; **p< 0.01; ***p< 0.001; ****p<0.0001. A, anterior; AL, anterolateral; AM, anteromedial; Au, auditory cortex; LI, interomediolateral; LM, lateromedial; M, medial; P, posterior; PM, posteromedial; POR, postrhinal; RL, rostrolateral; RS, retrosplenial cortex; S1, primary somatosensory cortex; V1, primary visual cortex.