Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-PSD-95 | Invitrogen | Cat# 51–6900, RRID:AB_2533914 |
| Goat anti-PSD-95 | Abcam | Cat# 12093, RRID:AB_298846 |
| Guinea Pig anti-Synapsin 1/2 | Synaptic Systems | Cat# 106004, RRID:AB_1106784 |
| Rabbit anti-Homer 1 | Synaptic Systems | Cat# 160006, RRID:AB_2631222 |
| Guinea Pig anti-vGlut2 | Synaptic Systems | Cat# 135404, RRID:AB_887884 |
| Mouse anti-Gephyrin | Synaptic Systems | Cat# 147011, RRID:AB_887717 |
| Mouse anti-Aβ HJ3.4 | (Bero et al., 2012) | N/A |
| Goat anti-Rabbit IgG-Alexa 594 | Invitrogen | Cat# A11037, RRID:AB_2534095 |
| Goat anti-Guinea Pig IgG-Alexa 488 | Invitrogen | Cat# A11073, RRID:AB_2534117 |
| Donkey anti-Goat IgG-Alexa 594 | Invitrogen | Cat# A11058, RRID:AB_2534105 |
| Mouse anti-NR2B | BD Biosciences | Cat# 610416, RRID:AB_397796 |
| Chicken anti-mGlur5 | Aves Labs | Cat# ER5, RRID:AB_2313544 |
| Streptavidin conjugated ATTO 490LS | ATTO-TEC | Cat# ATTO-490LS |
| Deposited Data | ||
| Spatial position of postsynaptic density in threedimensional electron microscopy data set. | Saturated Reconstruction of a Volume of Neocortex. Kasthuri et al., 2015 | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57/Bl6J | The Jackson Labs | RRID: IMSR_JAX:000664 |
| Mouse: Synaptobrevin-mRFP | Dr. Jens Rettig(Matti et al., 2013 | N/A |
| Mouse: PSD-95-Venus | Dr. Haining Zhong(Fortin et al., 2014 | N/A |
| Mouse: APP PS1 | The Jackson Labs(Borchelt et al., 1996; Jankowsky et al., 2001) |
RRID:MMRRC_034829-JAX |
| Mouse: APP NL-F | Dr. Takaomi C. Saido(Saito et al., 2014) |
RRID:IMSR_RBRC06343 |
| Mouse: PS19 P301S | The Jackson Labs(Yoshiyama et al., 2007 |
RRID:IMSR_JAX:008169 |
| Software and Algorithms | ||
| SEQUIN Matlab code for synapse analysis | This report | https://github.com/KummerLab/SEQUIN |
| ImageJ 1.51n | National Institutes of | https://imagej.nih.gov/ij/ |
| Health | ||
| Imaris 9 | Bitplane | https://imaris.oxinst.com/ |
| Matlab 2018b and 2019b | MathWorks | https://www.mathworks.com/products/matlab.html |
| Prism 8 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Excel | Microsoft | https://products.office.com/en-us/excel |
| R Studio | R Studio | https://rstudio.com/ |
| Other | ||
| Nanoruler | GATTAQUANT | Cat# Nanoruler SIM 140YBY |