Extended Data Fig. 5 |. Unoptimized mitoTALE–split DddA_tox fusions mediate modest editing of mitochondrial ND6 in HEK293T cells.

a, Architectures of non-UGI containing ND6-mitoTALE–DddA_tox fusion pair. DddA_tox was split at G1333 or G1397, with each half fused to either the left TALE or the right TALE. TALEs bind to mtDNA sequences (blue) that flank a 15-bp spacing region in mitochondrial ND6. Target cytosines are shown in purple. The last TALE repeat (*) did not match the reference genome⁹ (see Supplementary Table 4). b, mtDNA editing efficiencies of mitoTALE–DddA_tox pairs in the listed split orientations. The dashed line is drawn at 0.1%. Values and error bars reflect the mean ± s.d. of n = 3 independent biological replicates.