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. Author manuscript; available in PMC: 2021 Jan 8.
Published in final edited form as: Nature. 2020 Jul 8;583(7817):631–637. doi: 10.1038/s41586-020-2477-4

Extended Data Fig. 7 |. Stalling mtDNA replication impairs mitochondrial base editing in human cells.

Extended Data Fig. 7 |

a, Schematic of experimental design. Addition of doxycycline (Dox) induces the stable expression of a dominant-negative mutant of DNA polymerase-gamma containing a D1153A substitution (POLGdn) in a HEK293-derived cell line31. Total cell lysate was collected at indicated time points for western blotting of POLGdn in n = 3 independent biological replicates. b, mtDNA levels of uninduced (no Dox) and induced (+Dox) cells treated with indicated DdCBE 48 h post-transfection. mtDNA levels were measured by qPCR and normalized to uninduced cells without DdCBE treatment. c, Editing efficiencies of indicated DdCBE in uninduced and induced cells 48 h post-transfection. All values and error bars in b and c reflect the mean ± s.d. of n = 3 independent biological replicates.