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. 2020 Jul 7;11:1265. doi: 10.3389/fimmu.2020.01265

Figure 2.

Figure 2

Gene expression profile of pro- and anti-inflammatory molecules in VAT of WT and NOD2−/− mice fed a CTD or HFD. Relative expression of IL-12p35 (A), mMCP-4 (B), IL-4 (C), arginase-1 (D), IL-10 (E), and Foxp3 (F) in VAT by RT-PCR. Adipocyte hypertrophy was assessed in NOD2−/− and WT mice after 20 weeks on HFD or CTD (G–J) (original magnification 400x). (K) Morphometric quantification of adipocyte size in VAT of WT and NOD2−/− mice fed a CTD or HFD. The results are expressed as the mean ± SEM and are a compilation of 3 independent experiments (n = 4–6 per group). Asterisks represent statistically significant differences (*p < 0.05) compared to WT on CTD; (#p < 0.05) compared to WT on HFD; (&p < 0.05) compared to NOD2−/− mice on CTD. Significant differences between the groups were compared by one-way ANOVA followed by Tukey's multiple-comparison test.