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. 2020 Jul 7;11:1265. doi: 10.3389/fimmu.2020.01265

Figure 5.

Figure 5

Th1 and Treg response generation in MLN and cytokine profile in small intestine of WT and NOD2−/− mice fed a CTD or HFD. Relative expression of T-bet (A), IFN-γ (B), occludin (C) and claudin-2 (D) was assessed in NOD2−/− and WT mice after 20 weeks on HFD or CTD by RT-PCR. Percentage and absolute numbers of Treg (CD4+Foxp3+) (E) or Th1 cells (CD4+T-bet+) (F) were determined in MLNs by flow cytometry. Percentages of Treg or Th1 are shown in representative dot plots in lymphocyte gate (G,H). The results are expressed as the mean ± SEM and are a compilation of 3 independent experiments (n = 4–8). Asterisks represent statistically significant differences (*p < 0.05) compared to WT on CTD; (#p < 0.05) compared to WT on HFD; (&p < 0.05) compared to NOD2−/− mice on CTD. Significant differences between two groups were compared by Student's t-test followed Mann-Whitney test; and between more groups by one-way ANOVA followed by Tukey's multiple-comparison test.