Platelets and Plt-EVs promote hemostasis and reduce vascular permeability in in vivo mouse models. (A) Schematic outlining procedures for mouse tail snip assay and Miles assay. (B) Quantitation of blood collected from mice treated with Plts, Plt-derived EVs, and FFP. (C) Absorbance quantitation of Evans blue dye extravagated from challenged regions of the mouse. Bars in both graphs represent mean + SEM. *Indicates a significant difference from control. #Indicates a difference between treatment groups, as determined by one-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).