Table 3.

Primers and Probes for RT-PCR COVID-19 Diagnostics

Target	Country	Name	Type	Sequence (5’ → 3′)
N	US-CDC	2019-nCoV_N1-F	F	GAC CCC AAA ATC AGC GAA AT
		2019-nCoV_N1-R	R	TCT GGT TAC TGC CAG TTG AAT CTG
		2019-nCoV_N1-P	P	ACC CCG CAT TAC GTT TGG TGG ACC
		2019-nCoV_N2-F	F	TTA CAA ACA TTG GCC GCA AA
		2019-nCoV_N2-R	R	GCG CGA CAT TCC GAA GAA
		2019-nCoV_N2-P	P	ACA ATT TGC CCC CAG CGC TTC AG
		2019-nCoV_N3-F	F	GGG AGC CTT GAA TAC ACC AAA A
		2019-nCoV_N3-R	R	TGT AGC ACG ATT GCA GCA TTG
		2019-nCoV_N3-P	P	AYC ACA TTG GCA CCC GCA ATC CTG
	Charité Germany	N_Sarbeco_F	F	CAC ATT GGC ACC CGC AAT C
		N_Sarbeco_R	R	GAG GAA CGA GAA GAG GCT TG
		N_Sarbeco_P	P	ACT TCC TCA AGG AAC AAC ATT GCC A
	Chinese-CDC	CCDC-N-F	F	GGG GAA CTT CTC CTG CTA GAA T
		CCDC-N-R	R	CAG ACA TTT TGC TCT CAA GCT G
		CCDC-N-P	P	TTG CTG CTG CTT GAC AGA TT
	Hong Kong University	HKU-N-F	F	TAA TCA GAC AAG GAA CTG ATT A
		HKU-N-R	R	CGA AGG TGT GAC TTC CAT G
		HKU-N-P	P	GCA AAT TGT GCA ATT TGC GG
	NIID Japan	NIID_2019-nCOV_N_F2	F	AAA TTT TGG GGA CCA GGA AC
		NIID_2019-nCOV_N_R2	R	TGG CAG CTG TGT AGG TCA AC
		NIID_2019-nCOV_N_P2	P	ATG TCG CGC ATT GGC ATG GA
E	Charité Germany	E_Sarbeco_F1	F	ACA GGT ACG TTA ATA GTT AAT AGC GT
		E_Sarbeco_R2	R	ATA TTG CAG CAG TAC GCA CAC A
		E_Sarbeco_P1	P	ACA CTA GCC ATC CTT ACT GCG CTT CG
RdRp	Charité Germany	RdRp_SARSr-F	F	GTG ARA TGG TCA TGT GTG GCG G
		RdRp_SARSr-R	R	CAR ATG TTA AAS ACA CTA TTA GCA TA
		RdRp_SARSr-P2	P	CAG GTG GAA CCT CAT CAG GAG ATG C
ORF1	Chinese-CDC	CCDC-ORF1-F	F	CCC TGT GGG TTT TAC ACT TAA
		CCDC-ORF1-R	R	ACG ATT GTG CAT CAG CTG A
		CCDC-ORF1-P	P	CCG TCT GCG GTA TGT GGA AAG GTT ATG G
	Hong Kong University	HKU-ORF1-F	F	TGG GGY TTT ACR GGT AAC CT
		HKU-ORF1-R	R	AAC RCG CTT AAC AAA GCA CTC
		HKU-ORF1-P	P	TAG TTG TGA TGC WAT CAT GAC TAG
RNAse P (control)	US-CDC	RP-F	F	AGA TTT GGA CCT GCG AGC G
		RP-R	R	GAG CGG CTG TCT CCA CAA GT
		RP-P	P	TTC TGA CCT GAA GGC TCT GCG CG