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. 2020 Jun 8;5(8):987–994. doi: 10.1038/s41564-020-0733-x

Table 1.

Data inputs for an ad hoc committee naming uncultivated SAGs and MAGs

Category Parameters for data quality recommendations
Genome quality Percent completion
Percent contamination
Presence of 5S, 16S 23S rRNA gene (and level of completeness)
Number of tRNA genes
Assembly quality N50 (defined as the length of the shortest contig in the set of largest contigs that together constitute at least half of the total assembly size)
Number of contigs
Naming conventions Status of Candidatus and future nomenclature (for example, superscript u, c or e designating uncultivated, Candidatus or environmental microorganisms, respectively)
Description requirements Phenotype or metabolic prediction based on DNA sequence
Ecological and biogeographic consideration
Additional metadata (including guanine and cytosine content, genome size and number of protein coding genes)
Validation of MAG and SAG nomenclature Potential for informational system of classification, validation and DOI assignment