Table 3.
Antibodies used for Treg evaluation
| Step | Antigen | Conjugate | Clone | Vendor |
|---|---|---|---|---|
| Surface | β7 | PerCP/Cy5.5 | FIB504 | BioLegend |
| Surface | CCR7 | Alexa Fluor 488 | G043H7 | BioLegend |
| Surface | CCR9 | Brilliant Violet 421 | L053E8 | BioLegend |
| Surface | CD4 | Alexa Fluor 700 | OKT4 | BioLegend |
| Surface | CD8 | V500 | RPA-T8 | BD Biosciences |
| Surface | CD25 | Pe-Cy7 | 2A3 | BD Biosciences |
| Surface | CD45RA | Brilliant Violet 605 | HI100 | BioLegend |
| Surface | CD127 | Brilliant Violet 650 | A019D5 | BioLegend |
| Surface | Live-Dead | eFL780 | ThermoFisher Scientific | |
| Intracellular | CD3 | Brilliant Violet 785 | OKT3 | BioLegend |
| Intracellular | FoxP3 | PE | PCH101 | ThermoFisher Scientific |
| Intracellular | Helios | Alexa Fluor 647 | 22F6 | BioLegend |
| Intracellular | Ki67 | PE/Dazzle-594 | Ki67 | BioLegend |