Table 4.
Antibodies used for evaluation of T-cell activation
| Step | Antigen | Conjugate | Clone | Vendor |
|---|---|---|---|---|
| Surface | CD4 | Alexa-700 | RPA-T4 | eBioscience |
| Surface | CD8 | V500 | RPA-T8 | BD Biosciences |
| Surface | Live-Dead | eFL780 | ThermoFisher Scientific | |
| Intracellular | IL-2 | PE | MQ1-17H12 | BioLegend |
| Intracellular | TNF-α | PerCPCy5.5 | Mab11 | BioLegend |
| Intracellular | CD154 | Alexa-647 | 24–31 | BioLegend |
| Intracellular | IL-17 | Brilliant Violet 421 | TC11-18H10.1 | BioLegend |
| Intracellular | IFN-γ | Brilliant Violet 650 | 4S.B3 | BioLegend |
| Intracellular | CD3 | Brilliant Violet 785 | OKT3 | BioLegend |