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. 2020 Jun 25;21:523–526. doi: 10.1016/j.omtn.2020.06.019

Table 1.

Summary of the CRISPR-Induced Mutant Rabbit Lines in This Study

Method System Rabbits Lines Gene Positive Mutation Rabbits Rabbit with Large Fragment Mutation (% Positive Rabbits) Rabbits without Large Fragment Mutation (% Positive Rabbits)
Cas9 SpCas9 F1 (Figure S1) F8 7 7 (100) 0
F2 (Figure S2) F9 6 2 (33.3) 4 (67.7)
S1 (Figure S3) Sost 5 3 (80.0) 2 (20.0)
F3 (Figure S4) Fbn1 7 6 (85.7) 1 (14.3)
G1 (Figure S5) Ggta 7 2 (28.6) 5 (71.4)
S2 (Figure S6) Sry 5 4 (80.0) 1 (20.0)
xCas9 T1 (Figure S7) Tyr 4 2 (50.0) 2 (50.0)
T2 (Figure S8) Tyr 7 4 (57.1) 3 (42.9)
BEs BE4 D1 (Figure S9) Dmd 7 0 7 (100)
M1 (Figure S10) Mstn 7 0 7 (100)
T3 (Figure S11) Tia1 7 0 7 (100)
T4 (Figure S12) Tyr 7 0 7 (100)
ABE D2 (Figure S13) Dmd 7 0 7 (100)
S3 (Figure S14) Sod1 7 0 7 (100)
xCas9-BE4 T5 (Figure S15) Tyr 7 0 7 (100)
xCas9-ABE T6 (Figure S16) Tyr 7 0 7 (100)