Figure 7.

Knockdown of YY1 Attenuated Breast Cancer Cells Resistance to Adriamycin Treatment through Reducing Cells Stemness

(A and B) Stemness marker expression was examined by western blot and quantitative real-time PCR in MCF-7/Adr cells with or without YY1 knockdown; samples derive from the same experiment and blots were processed in parallel. (C–E) IC₅₀ values of adriamycin in MCF-7/Adr cells with or without YY1 knockdown. (F and G) CD44⁺/CD24⁻ population or spheroid formation (50X) measured in cells depicted in (A). The data are presented as the mean ± SD, n ≥ 3, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 versus MCF-7 or NC.