TABLE 4.
Multicriteria table indicating the score of each primer set for the three assessment steps performed
| Analyses | Criteria | MG2 | MG2fwh | MG2ANML‐degen | fwh1 | fwh2 | fwhFol | Lep1 | mlHCO | Hex | Zeale | Epp‐degen | Epp | Score details | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| In silico | Mean amplification success | 3 | 3 | 3 | 3 | 2 | 3 | 1 | 1 | 3 | 1 | 3 | 1 | ≥100 | ]50:100[ | ≥50 |
| Mean penalty | 3 | 3 | 3 | 3 | 2 | 3 | 1 | 1 | 3 | 1 | 3 | 1 | ≤50 | ]50:80[ | ≥80 | |
| In vivo—mock communities | Bat identification | 3 | 3 | 3 | 3 | 3 | 3 | 1 | 3 | 1 | 1 | 3 | 2 | 0 PCR/3 | 2 PCRs/3 | 3 PCRs/3 |
| Arthropod taxa detection success | 3 | 3 | 3 | 3 | 3 | 3 | 1 | 3 | 3 | 1 | 3 | 2 | ≤80% | ]80%: 90%[ | ≥90% | |
| Identification at the species level | 3 | 3 | 3 | 3 | 3 | 3 | 2 | 3 | 3 | 2 | 1 | 1 | ≤10 taxa | ]10:15[ | ≥15 | |
| In vivo—guano | Bat identification | 3 | 3 | 3 | 3 | 3 | 3 | 2 | 3 | 1 | 1 | 3 | 3 | 0 | 1 sp | 2 sp |
| Occurrence of prey taxa | 2 | 2 | 2 | 3 | 2 | 1 | 1 | 1 | 1 | 3 | 1 | 1 | ≤100 | ]100:130[ | ≥130 | |
| Number of orders | 2 | 2 | 2 | 2 | 3 | 2 | 2 | 2 | 2 | 3 | 2 | 2 | ≤5 | ]5:10[ | ≥10 | |
| Number of families | 2 | 3 | 2 | 3 | 3 | 1 | 2 | 1 | 1 | 3 | 1 | 1 | ≤25 | ]25:30[ | ≥30 | |
| Number of genus | 2 | 2 | 2 | 3 | 2 | 2 | 1 | 2 | 1 | 3 | 1 | 1 | ≤50 | ]50:70[ | ≥70 | |
| Number of species | 2 | 2 | 2 | 3 | 2 | 1 | 2 | 1 | 1 | 3 | 1 | 1 | ≤60 | ]60:80[ | ≥80 | |
| Cumulative score | 28 | 29 | 28 | 32 | 28 | 25 | 16 | 21 | 20 | 22 | 22 | 16 | ||||
Scores range from the less efficient (“1” represented in red) to the most efficient (“3” represented in green).