Table 1.

Standard membrane feeding assay results for Pfs230D1-specific mAbs.

mAb	Isotype	Exp No.	TB activity with human complement
			Inf/Dissa	Avg No. oocysts	Median No. oocysts (range)	% reduction oocysts	% reduction prevalence
3G2	IgG2b	1	20/20	59.6	62 (21–110)	16.4	0
		6	21/21	62.8	58 (14–139)	−0.7	0
5G3	IgG1	1	20/20	68.6	66 (20–115)	3.7	0
		6	21/21	47.9	39 (8–99)	23.2	0
5H1	IgG1	1	2/20	0.1	0 (0–1)	99.9	90
		2	0/20	0	0 (0–0)	100	100
		3	15/20	1.8	1 (0–6)	93.2	25
		6	21/22	4.5	4 (0–13)	92.9	4.6
7G4	IgG1	6	21/21	74.2	68 (6–214)	−19	0
		7	20/20	46	47.5 (1–83)	9.3	0
4F12b	IgG1	1	20/20	39.4	38 (8–76)	42.2	0
		2	15/20	8.4	0 (0–38)	67.1	25
4F12(3B6)		4	21/21	59.6	55 (4–118)	40.3	0
		6	22/22	33.8	30 (1–64)	45.9	0
rh4F12b	mFab/	5	17/20	5.4	3.5 (0–27)	92.2	15
	hIgG1 Fcc	8	19/20	8.1	5.5 (0–19)	84.3	5

m murine, h human, TB transmission-blocking.

^aInfected/Dissected.

^bPercent reduction in oocysts by rh4F12 compared to 4F12 is significantly different (p = 0.0059, by Welch Two Sample t-test); mAbs were tested at ~1.0 mg/mL IgG except Exp. 8 which was assessed at 0.6 mg/mL.

^cPositive and negative controls for this assay are shown in Supplementary Table 2.