Skip to main content
. 2020 Jul 24;11:3734. doi: 10.1038/s41467-020-17544-3

Fig. 2. USA expression in the thymus of CRP and INS2 Tg mice support negative selection of antigen-specific MHC class I-restricted T cells.

Fig. 2

a Representative flow plot and b relative ratio of OT-I:control thymocytes 24 h after overlay on the indicated thymic slices. OT-I:WT control ratios were normalized to the littermate control (LMC) thymus (mean ± SEM, n = 72 LMC, 44 CRP, 12 CRPlo, 26 INS2, 10 INS2lo). c CD8+ SP cells among live TCRβhi OT-I cells 24 h after overlay on the indicated thymic slices. (mean ± SEM, n = 100 LMC, 48 CRP, 18 CRPlo, 33 INS2, 10 INS2lo). d Relative ratio of OT-I:control thymocytes 24 h after overlay on the indicated thymic slices (mean ± SEM, n = 21 LMC CRP, 17 Aire+/+ CRP, 15 Aire−/− CRP, 12 INS2 LMC, 6 Aire+/+ INS2, 12 Aire−/− INS2). e, f LMC, CRP and INS2 Tg mice (CD45.2) were irradiated and reconstituted with 1% OT-I (CD45.1) and 99% WT (CD45.1.2) bone marrow. Analysis of the proportion of mature TCRβhi CD8+ SP OT-I thymocytes among total live cells and absolute cell number of CD8SP OT-I in the thymus (e) (mean ± SEM, n = 20 LMC, 11 CRP, 13 INS2, from 3 independent experiments) and proportion of OT-I T cells among live CD8+ T cells and absolute OT-I cell number in the peripheral lymph nodes (f) of low-density bone marrow chimeras 6–8 weeks post-reconstitution (mean ± SEM, n = 12 LMC, 7 CRP, 8 INS2, from 2 independent experiments). All the p-values indicated are calculated by Kruskal–Wallis test followed by Dunn’s post-hoc comparisons to LMC, two-sided. *p < 0.05, **p < 0.01, ***p < 0.001 relative to LMC. Source data are provided as a Source Data file.