Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 24;11:3734. doi: 10.1038/s41467-020-17544-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Representative flow plots of CD4 and CD8 on TCRβ^hi OT-I thymocytes, and b proportion of CD4⁻CD8⁻ cells among live TCRβ^hi OT-I thymocytes 24 h after overlay on the indicated thymic slices (mean ± SEM, n = 70 LMC, 30 CRP, 12 CRP^lo, 27 INS2, 12 INS2^lo). c Representative flow plot of PD-1 and CD122 and d proportion of CD122⁺PD-1⁺ cells among CD4⁻CD8⁻TCRβ^hi OT-I T thymocytes 24 h after overlay on the indicated thymic slices (mean ± SEM, n = 30 LMC, 24 CRP, 18 INS2). e–l Littermate control (LMC), CRP, CRP^lo and INS2 Tg mice (CD45.2) were irradiated and reconstituted with 1% OT-I (CD45.1) and 99% WT (CD45.1.2) bone marrow. OT-I cells in the indicated hosts were analyzed. e Representative flow plot of CD4 and CD8 expression on TCRβ^hi OT-I thymocytes. f Proportion of CD4⁻CD8⁻ cells within the TCRβ^hi OT-I thymocyte population and absolute number of PD1⁺CD122⁺TCRβ^hi CD4⁻CD8⁻ OT-I thymocytes (mean ± SEM, left panel: n = 8 LMC, 8 CRP, 7 INS2, right panel: n = 8 LMC, 7 CRP, 8 INS2). g Proportion and cell number of OT-I T cells of TCRαβ intraepithelial lymphocytes (IEL) (mean ± SEM, left panel: n = 8 LMC, 8 CRP, 7 INS2, right panel: n = 10 LMC, 8 CRP, 8 INS2). h Representative flow plot of CD8α and CD8β staining on the OT-I T cells. i The proportion and cell number of TCRαβ⁺ CD8αα IEL among the OT-I T cells (mean ± SEM, left panel: n = 8 LMC, 8 CRP, 7 INS2, right panel: n = 10 LMC, 8 CRP, 8 INS2). j Proportion of OT-I T cells within the live TCRαβ⁺ IEL. k Proportion of TCRαβ⁺ CD8αα of the OT-I T cells. l Proportion of mature TCRβ^hi CD8⁺ SP OT-I thymocytes among total live cells in the thymus (mean ± SEM, n = 9 mice per group). Data are from at least two independent experiments. The p-values for panels b, d, f, g and i were calculated by Kruskal–Wallis test followed by Dunn’s post-hoc comparisons to LMC, two-sided. For panels j-l a two-tailed Mann-Whitney U test was performed. *p < 0.05, ***p < 0.001 relative to LMC. Source data are provided as a Source Data file.