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. 2020 Jul 24;11:3709. doi: 10.1038/s41467-020-17466-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a A representative micrograph of a 15 e⁻/Å², 0° tilt image (left) shown together with the first 2 e⁻/Å² of the same image (right). Scale bar: 100 nm. b The detected Thon rings for the two images in (a). The white arcs represent the maximum resolution reported by Gctf³⁸. c Relationship between the maximum frequencies used for defocus determination (X-axis) and the precision of defocus estimation (Y-axis). Grey line: error in defocus determination with limited frequencies compared to the defocus determination with the full range of frequencies; blue line: maximal resolution up to which the estimated CTF fits with the data, as reported by Gctf³⁸.