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. 2020 Jul 24;11:3709. doi: 10.1038/s41467-020-17466-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–c Structures of TMV (a, b) and the corresponding FSC curves (c) as processed by the conventional subtomogram averaging workflow. The dataset in a was recorded using a conventional dose-symmetric scheme and in b using the proposed hybrid scheme. Note the “halos” observed next to the density in e, f and marked with the asterisks. d–g Processing of a larger dataset of hybrid tomograms of TMV: d conventional StA map; e Hybrid map, reconstruction only from the high-dose image; f and the refined map with the refined CTF; g the corresponding FSC curves. Scale bars: 10 nm.