Figure 2.

a-wave amplitudes of scotopic ERG responses were reduced in the BTBR mice. (A) Dark-adapted intensity responses. Examples of traces recorded at increasing flash strengths under no light background. Flash onset is at 20 ms. Bandpass is at 0.3–300 Hz. (B–G) Measurements of intensity responses are provided for a-wave amplitudes (B) and implicit times (C) as well as b-wave amplitudes (E) and implicit times (E), and for the b-wave to a-wave (b/a) amplitude ratios at 1.36 log cd∙s m⁻² stimulus strength (F). a-wave amplitudes at 7 ms post-stimulus are shown in (G). Amplitudes were measured at 7 ms to exclude post-synaptic contribution to the a-wave. (H–L) The ratios of ERG responses elicited by a stimulus of 1.36 log cd∙s m⁻², at time 0 and 10 min following transition from light (30 cd m⁻²) to dark background, are provided for the following parameters: a-wave amplitudes (H) and implicit times (I); b-wave amplitudes (J) and implicit times (K); and for b/a amplitude ratios (L). n = 6 B6 male mice, n = 7 BTBR male mice. For ERG intensity/response functions, data from B6 and BTBR mice were compared using two-way repeated-measures ANOVA, followed (if P < 0.05) by Sidak post-hoc pairwise comparison. In panel (B) and (G), P < 0.01 (B) and P < 0.05 (G) for two-way repeated-measures ANOVA comparing a-wave amplitudes between B6 and BTBR mice; *indicates P < 0.05, ^#indicates P < 0.01, and ⁺indicates P < 0.0001 for post-hoc pairwise comparison. For ERG dot plots, the data were compared using the non-parametric Mann–Whitney U test. Data represent mean ± SEM. IT implicit time.