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. 2020 Jul 24;10:12422. doi: 10.1038/s41598-020-69068-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative transcript expression of three stress response genes in T. castaneum following micro-injection with PAMAM-CNTs and dsRNA. T. castaneum larvae were injected with 50 µg/mL naked PAMAM-CNTs or 50 µg/mL PAMAM-CNT-dsRNAαtub and expression measured at 96 h, along with control (saline), dsRNAαtub and dsRNA GFP. Asterisks represent significant differences between untreated and treated larvae (P < 0.001). The data are presented as means ± SEM of three replicate samples.