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. 2020 Jul 24;10:12422. doi: 10.1038/s41598-020-69068-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Frequency of blistered wing phenotype in T. castaneum pupae. No phenotype was seen in T. castaneum larvae injected with saline (control) or naked PAMAM-CNTs after pupation. A blistered phenotype was observed, post-pupation, in larvae injected with dsRNA_αtub or PAMAM-CNT-dsRNA_αtub exposed. The phenotype was significantly more frequent in the PAMAM-CNT-dsRNA_αtub injected group compared to dsRNA_αtub alone (P = 0.016). The data are presented as means ± SEM (n = 41 per treatment). Different letters indicate a significant difference in survival between treatments (a > b > c).