Table 1.
Oligos for dsRNA preparation and target transcript evaluation.
| Transcript | Function | NCBI ID | Oligo sequence | Size (bp) | Tm (°C) |
|---|---|---|---|---|---|
| α-tubulin | Cytoskeletal microtubule component | XP_966492 |
Tc-dsRNAαtub_F: CAAGGAAATCGTCGACTTGG Tc-dsRNAαtub_R: TGAAGGCACAGTCAGAATGC Tc-qPCR_atub_F: AAGACGCCGCCAATAACTAC Tc-qPCR_atub_R: TCGGCCAATTTACGGATG |
275 91 |
51.8 51.8 |
| mtpol | Mitochondrial RNA polymerase | XM_962507.1 |
Tc-dsRNAmtpo_F: GAATTACTCGACACAATCC Tc-dsRNAmtpo_R: CTGATTGTGGAAGATGAGG Tc-qPCR_mtpo_F: ATGTGCTCCACCTTGCAAAC Tc-qPCR_mtpol_R: TCAAATCCTTGCCTCTGGT |
219 95 |
56.3 58.5 |
| Tc12580, Dronc | Caspase initiator | NM_001170644.1 |
F: TCGGAGAAGGAGTACAAG R: TATCCACTCTTCCTCCAC |
126 | 50.9 |
| TcNc-like, Dredd | Caspase initiator | XM_015979881.1 |
F: ACTCCACTGACACCATAGAC R: GGACATAACTTGGTCCAC |
77 | 51.4 |
| Tcinhib apop1, Buffy | Apoptotic inhibitor | XM_961548.4 |
F: AACTGTTGAGGTGTGAGC R: CTATCCTCCAACAACTCC |
103 | 51.0 |
| GFP | Green fluorescent protein | MN114103.1 |
F: CCATCTAATTCAACAAGAATTGGGAC R: GGTCCTTCTTGAGTTTGTAAC |
796 | 51.8 |
| RPS3 | Ribosomal protein of T. castaneum | XM_008194244.2 |
F: ACCTCGATACACCATAGCAAGC R: ACCGTCGTATTCGTGAATTGAC |
60 | 50.5 |
Tm is annealing temperature of oligos.