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. 2020 Jul 24;10:12392. doi: 10.1038/s41598-020-69122-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow velocity in pial arterioles after dMCAO and RIPerC. (a) Mean RBC velocity of pial vessels from control (sham treated) and RIPerC treated aged rats (bars show mean ± SD). Two-way ANOVA confirmed a significant main effect of Time and a trend towards a main effect of Treatment on flow velocity in measured segments. To better illustrate diameter changes due to stroke and treatment, (b) shows flow velocity data normalized to values measured prior to dMCAO. (c) shows flow velocity normalized to values measured 60 min after ischemic onset (i.e. post dMCAO but pre-RIPerC or sham treatment). Individual means for each animal are plotted, and red lines show the group mean. For both (b) and (c), two-way ANOVAs demonstrated no significant main effects of Time or Treatment and no significant Time × Treatment interactions.