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. 2020 Jun 26;41:101045. doi: 10.1016/j.molmet.2020.101045

Figure 7.

Figure 7

Acute treatment with phanginin A attenuated gluconeogenesis by inhibiting the cAMP signaling pathway by activating SIK1 and PDE4 in ob/ob mice. Mice were treated as described in Methods. (A) Blood glucose levels in the pyruvate tolerance test of ob/ob mice were measured. (B) Hepatic SIK1 phosphorylation in ob/ob mice was detected. (C) PDE4 activity and (D) cAMP levels in the liver of ob/ob mice were detected. (E) CREB phosphorylation in the liver of ob/ob mice was analyzed by Western blotting. (F) Hepatic gluconeogenic gene expression in ob/ob mice were evaluated. (G) Glycogen content was measured in the liver of ob/ob mice. All of the results are presented as the mean ± SEM (n = 8 for all of the groups, except for n = 6–7 in Figure A). ∗P < 0.05 and ∗∗P < 0.01 vs the vehicle group.