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Characteristic motif in open-reading frame 3a (Orf3a). A: characteristic LXXC motif in Orf3a from multiple sequence alignment of reported strains. The LWLC is associated with viral expression, whereas CWLC is the motif described in CoV-1. B: conserved CoV-1 sequences of Orf3b. The header of alignment shows the amino acid positions highlighting nonconsensus sequences.
