Figure 5.

AIFM2 silencing ameliorates inflammation and multiple organ injury in SAP. (A) Histological alterations, MPO expression, acinar cell apoptosis, and microstructural changes in pancreatic tissues from PRSS1^Tg SAP mice with shAIFM2-mediated AIFM2 silencing were measured by H&E, immunohistochemical staining, TUNEL assays, and TEM, respectively. (B) Histological and ultrastructural evaluation of lung, liver, and kidney tissues from PRSS1^Tg SAP model mice treated with shAIFM2. Black arrows (↑): cell nuclei; white arrows (↑): endoplasmic reticula; blue arrows (↑): zymogen granules; purple arrows (↑): mitochondria; red arrows (↑): apoptotic bodies; orange arrows (↑): lamellar bodies. The levels of ALT, AST (C), CR and urea (D) in serum from PRSS1^Tg mice treated with shAIFM2 or shCON. (E) Expression of IL-1β, IL-6, and TNF-α in serum were measured by ELISA from PRSS1^Tg mice treated with shAIFM2. The data are presented as the means ± SDs; ns, no significant difference; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. Scale bars (H&E, MPO) = 100 µm; scale bars (TUNEL) = 200 µm.