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. 2020 Jul 9;10(18):8298–8314. doi: 10.7150/thno.46934

Figure 7.

Figure 7

ATF6 binds to the promoters of p53 and AIFM2 in acinar cells. (A) Binding of ATF6 to p53 and AIFM2 promoter region in vitro by chromatin immunoprecipitation (ChIP) using anti-ATF6 or anti-IgG antibodies in untreated (-) or caerulein-treated (+) pancreatic acinar cells of PRSS1Tg mice. (B) Input and immunoprecipitated DNA purified by ChIP were measured using qRT-PCR. (C) Schematic of deletion mutants from p53 and AIFM2 promoters. The predicted ATF6 binding sites were marked as Site A, B and C. (D) Luciferase reporter studies on the p53 and AIFM2 promoter in 293T cells. Cells were co-transfected with a series of mutants of p53 or AIFM2 promoter plasmid and either Atf6 overexpression plasmids or the control plasmids. Luciferase activities was measured 48h later. (E) A mechanistic figure for regulation of ATF6 on p53-AIFM2 pathway through apoptosis in SAP. The data are presented as the means ± SDs; ns, no significant difference; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.