Figure 6.

Ablation of CD4⁺ T-cells contributes to skewed polarization of the myocardial macrophages away from the pro-fibrotic M2-like signature after injury. (A) Schematic diagram showing the experimental design. (B) Flow cytometric analysis showing M1- or M2-like macrophages among total PI^-F4/80⁺ macrophages at day 7 after CI to the P8 heart. Quantifications of (B) in terms of (C) %F4/80⁺ cells and (D) %Ly6C^hi M1- or (E) %Ly6C^loCD206⁺ M2-like cells among total F4/80⁺ cells harvested from the non-depleted (control) or CD4⁺ T-cell depleted (GK1.5) hearts, respectively. (F) qRT-PCR showing M2-like macrophage-associated genes of total F4/80⁺ cells purified from the control and GK1.5-treated hearts, respectively, by flow cytometry. (C-F) Data are presented as mean±S.E.M., *P<0.05, n=4-5 per group.