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. 2020 Jun 29;10(18):8018–8035. doi: 10.7150/thno.42943

Table 1.

Distribution of cell number and percentage of CD3+ T-cells in each of the six subsets as determined by t-SNE. At day 7 after CI, we purified CD45+CD3+ T-cells from the hearts of ICR mice that underwent CI at P3 and P8 by flow cytometry and captured the transcriptome of about ~1,123 and ~2,167 cells by scRNA-seq, respectively. Cells were filtered out if their mRNA contents were above or below two standard deviations of the mean value; if they showed high mitochondrial contents as demonstrated by fractions of mitochondrial transcripts > 10%; or if they expressed B cell specific markers Ebf1, Cd79a and Cd79b. After that, ~1,000 cells were randomly selected from each sample for further analysis.

Total P3 P8
After filtered 3290 1123 2167
Randomly selected 2000 1000 1000
S1 1437 764 (76.4%) 673 (67.3%)
S2 190 100 (10.0%) 90 (9.0%)
S3 157 65 (6.5%) 92 (9.2%)
S4 105 3 (0.3%) 102 (10.2%)
S5 78 43 (4.3%) 35 (3.5%)
S6 33 25 (2.5%) 8 (0.8%)