Distribution of cell number and percentage of CD3+ T-cells in each of the six subsets as determined by t-SNE. At day 7 after CI, we purified CD45+CD3+ T-cells from the hearts of ICR mice that underwent CI at P3 and P8 by flow cytometry and captured the transcriptome of about ~1,123 and ~2,167 cells by scRNA-seq, respectively. Cells were filtered out if their mRNA contents were above or below two standard deviations of the mean value; if they showed high mitochondrial contents as demonstrated by fractions of mitochondrial transcripts > 10%; or if they expressed B cell specific markers Ebf1, Cd79a and Cd79b. After that, ~1,000 cells were randomly selected from each sample for further analysis.