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. 2020 Jul 9;10(18):8281–8297. doi: 10.7150/thno.47315

Figure 1.

Figure 1

p68 represses Pkd1 expression by binding to the Pkd1 promoter. (A) The expression levels of Pkd1, Pkd2 and p68 were analyzed by qRT-PCR in mIMCD3 cells transfected with p68-siRNA or Negative Control (NC)-siRNA for 24 h. (B) The expression levels of p68 and PC1 were analyzed by Western blot in mIMCD3 cells transfected with p68-siRNA or NC-siRNA for 24 h. (C, D) ChIP and ChIP-qPCR assay was performed with anti-p68 antibody or normal mouse IgG in mIMCD3 cells. Anti-RNA polymerase II antibody was used as a positive control. Negative control (NC) primers were located about 2000 bp upstream of the transcription start site (TSS) of mouse Pkd1 gene. (E, F) ChIP and ChIP-qPCR assay was performed with anti-p68 antibody or normal mouse IgG in RCTE cells. Anti-RNA polymerase II antibody was used as a positive control. Negative control (NC) primers were also located about 2000 bp upstream of the transcription start site (TSS) of human PKD1 gene. (G) Alignment of Pkd1 promoter among species identified a consensus sequence, GGGCGGAGC, which may be a p68 binding motif.