Figure 3.

p68 decreases Pkd1 gene expression in response to oxidative DNA damage. (A) qRT-PCR analysis was performed to detect mRNA levels of p68 and Pkd1 in mIMCD3 cells treated with H₂O₂ at the indicated concentrations for 6h. (B) Western blot analysis was performed to detect protein levels of p68, PC1, γH2AX and H2AX in mIMCD3 cells treated with H₂O₂ at the indicated concentrations for 6h. (C) Immunofluorescent staining of γH2AX in mIMCD3 cells transfected with p68 siRNA or control siRNA following the treatment with or without H₂O₂ (0.5 mM) for 6 h. Scale bar, 50 µm. (D) Quantification of γH2AX staining intensity per nucleus in mIMCD3 cells transfected with p68 siRNA or control siRNA following the treatment with or without H₂O₂ for 6 h (100 cells scored per condition in three independent experiments). (E, F) qRT-PCR analysis was performed to detect mRNA levels of p68 and Pkd1 in mIMCD3 cells transfected with p68 siRNA or control siRNA and then treated with or without H₂O₂ (0.5 mM) for 6h.