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. 2020 Jul 9;10(18):8281–8297. doi: 10.7150/thno.47315

Figure 4.

Figure 4

p68 regulates Pkd1 expression by miR-182-5p mediated post-transcriptional repression. (A) The interaction between Drosha and p53 (top panel) and between Drosha and p68 (middle panel) was detected in mIMCD3 cells with anti-Drosha antibody followed by blotting with p68 and p53. IgG was used as a negative control. (B to D) The expression of miR-182-5p, miR-17 and miR-200c, pre-miR-182-5p, pre-miR-17 and pre-miR-200c, and pri-miR-182-5p, pri-miR-17 and pri-miR-200c in mIMCD3 cells transfected with p68 or control siRNA examined by qRT-PCR analysis. (E, F) The levels of Pkd1 mRNA and PC1 protein in mIMCD3 cells treated with a miR-182-5p inhibitor examined by qRT-PCR and Western blot analysis. (G, H) The levels of Pkd1 mRNA and protein were examined by qRT-PCR (G) and Western blot analysis (H) in mIMCD3 cells treated with a miR-182-5p mimics.